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Research Article Open Access

In Vitro antibacterial studies of phytosynthesized silver nanoparticles using Dianthus Caryophyllus L. (Carnation)

Rachna1*, Baljeet S. Saharan2, Mahender S. Yadav1, Nisha Sharma2

 

 

 

1Department of Physics,Kurukshetra University, Kurukshetra, 136119, India

2Department of Microbiology, Kurukshetra University, Kurukshetra, 136119, India.

Adv. Mater. Proc., 2018, 3 (1), 54-57

DOI: 10.5185/amp.2018/712

Publication Date (Web):01 January 2018

Copyright © IAAM-VBRI Press

Abstract


Abstract

A simple and efficient synthesis of silver nanoparticles (AgNPs) is reported here using red Dianthus caryophyllus flower, acting both as reducing and capping agent. The resultant silver colloids were characterized using UV-visible spectrophotometer, X-ray Diffractometer (XRD) and Transmission electron microscope (TEM). The surface absorption plasmon response and kinematics of reduction of silver ions were observed by UV-visible spectroscopy. The crystalline fcc structure of AgNPs was confirmed by its XRD pattern. Their morphological study was done with TEM, showing spherically shaped AgNPs in the range 10-20 nm. The antibacterial action was also studied using Agar well diffusion method against pathogenic bacteria cultures (Staphylococcus aureus, Bacillus cereus and Escherichia coli). AgNPs showed better antimicrobial activity against S. aureus culture. Copyright © 2018 VBRI Press.

Keywords


Nanobiotechnology, green synthesis, Dianthus caryophyllus, silver nanoparticles, antibacterial activity.