Research Article Open Access
In Vitro antibacterial studies of phytosynthesized silver nanoparticles using Dianthus Caryophyllus L. (Carnation)
Rachna1*, Baljeet S. Saharan2, Mahender S. Yadav1, Nisha Sharma2
1Department of Physics,Kurukshetra University, Kurukshetra, 136119, India
2Department of Microbiology, Kurukshetra University, Kurukshetra, 136119, India.
Adv. Mater. Proc., 2018, 3 (1), 54-57
DOI: 10.5185/amp.2018/712
Publication Date (Web):01 January 2018
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Abstract
A simple and efficient synthesis of silver nanoparticles (AgNPs) is reported here using red Dianthus caryophyllus flower, acting both as reducing and capping agent. The resultant silver colloids were characterized using UV-visible spectrophotometer, X-ray Diffractometer (XRD) and Transmission electron microscope (TEM). The surface absorption plasmon response and kinematics of reduction of silver ions were observed by UV-visible spectroscopy. The crystalline fcc structure of AgNPs was confirmed by its XRD pattern. Their morphological study was done with TEM, showing spherically shaped AgNPs in the range 10-20 nm. The antibacterial action was also studied using Agar well diffusion method against pathogenic bacteria cultures (Staphylococcus aureus, Bacillus cereus and Escherichia coli). AgNPs showed better antimicrobial activity against S. aureus culture. Copyright © 2018 VBRI Press.
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